I think I forgot to mention that they're worldspace UI items for a VR game I''m making. There are event triggers on each button so I'll try to recreate the system that you've used in your ping demo menu and see what happens. Edit: When I remove the event triggers on the buttons in the editor they get re-added to the buttons when the game starts. It's an odd issue and doesn't occur in your other scenes. Edit 2: I've noticed in the ping demo menu that setting it to use the gamepad gives you the option to change the deadzone of the controller whereas my version of the menu does not. Is it possible that this is the cause of the issue?