I have a problem with my game build, when I try to use it, it stops to work

I am using unity 2020.3.25f1, my target platform is WebGL, and I published a demo of my game on itch.io as a ZIP file that will be read by the browser, when I am trying to use it appears this error message
, honestly, what can I do to fix it?
I don't know what it means and how to fix it, however, I also use some external assets in my game, I can't remove it because are important for the game, but I also tried to fix them