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You can use the profiler with builds, just make sure Development Build is toggled (then run the build, and open the Profiler window in the editor and use the Active Profiler drop down).

Profiling is your best bet to tell you where your problem lies, but it does sound like a Garbage Collector issue.

 

Not sure there's enough info there for determining the cause. It would be good to mention Unity version, forward or deferred, DX11, 2D / 3D, HQ desktop or mobile-friendly, etc.

But based only on the idea that it works well in editor but not as an .exe ... I'm not sure what that could be. If you're running a HQ desktop game, I'd be thinking of heavy shaders and big texture loading, lighting / shadows, reflections (like from water), stuff like that.

If you can't get a definite idea about what's going wrong, as part of the guessing game you can try some things as test / rule-outs, like a shader warmup.

Anyway, if you are still stuck, post more info to help with the diagnosis.

And if you do figure it out, please let us know what it was.

 

Ah, yes, might be a 'uploading-alot-at-once-to-the-GPU'-issue. A related issue is that sometimes drivers/etc doesn't actually upload stuff to the GPU until the first time it is supposed to be drawn, this can be fixed by rendering to a RenderTexture.