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This happens to me periodically in Unity 2019.3.0b1. Restarting Unity has fixed it so far.
I kept having this problem in 2019.3.0b1. I changed my output directory to an external drive although it was still NTFS. I also quit saving over...
One caveat seems to be that post processing disables the transparency. I couldn’t get the above working until I disabled the post process on the...